Description
abm | FLAG-SNAIL Stable Expressing HEK293 Cell Line | T6290
The SNAIL family have been associated with EMT (epithelial-mesenchymal transition) events, and tumour progression. FLAG-SNAIL Stable Expressing HEK293 Cell Line was developed through stable transfection with the flag-tagged, full-length AJUBA cDNA in the pcDNA3.1 vector into HEK293 cells. The transfected cells were selected with G418. The cells may be used to further study SNAIL and its association with tumor development and progression.
Biosafety:
II
Organism:
Human (H. sapiens)
Source Organ:
Kidney
Growth Properties:
Adherent
Morphology:
Epithelial-like
Clones:
N/A
Passage Number:
N/A
Population Doner:
30 - 40 hours
Seeding Density:
30,000 - 50,000 cells/cm2
Markers:
G418 -resistant, Flag-SNAIL
Applications:
For Research Use Only
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm
Preservation:
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
1) Western blot
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C