abm | FLT3 Stable Ba-F3 Cell Line | T3080

abm | FLT3 Stable Ba-F3 Cell Line | T3080

Fusing a kinase domain to a homodimerization domain such as the PNT domain of ETV6 can result in activated kinase. The FLT3 Stable Ba/F3 Cell Line expresses activated FLT3 tyrosine kinase that is linked to a GFP reporter (GFP-ETV6-FLT3 in MSCV-puro vector). Ba/F3 cells expressing activated tyrosine kinase become completely dependent on the kinase activity for survival and as a result, the cells can be propagated even in the absence of IL-3.

Biosafety:

I

Organism:

Mouse

Source Organ:

Bone Marrow

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

12 - 22 hours

Seeding Density:

100,000 - 120,000 cells/ml; Split ratio: 1:2

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

FLT3 tyrosine kinase (GFP-ETV6-FLT3) via retroviral transduction

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

N/A

Quality Control:

WB

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C