abm | Gnpat Stable Mouse Myoblast (161) Cell Line | T6280

abm | Gnpat Stable Mouse Myoblast (161) Cell Line | T6280

Cells were isolated from cross-breed pups of Gnpat wildtype mice (Gnpat ^+/+) crossed with SV40 carrying Tg(H2‐K1‐tsA58)11Kio mice to obtain the Gnpat Stable Mouse Myoblast (161) Cell Line. This cell line is a Gnpat wildtype, which may be used alongside with Gnpat knockout cells for study (available at abm,

Biosafety:

II

Organism:

Mouse (M. musculus)

Source Organ:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Radial branching

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 36 hours

Seeding Density:

N/A

Markers:

Gnpat, no drug resistance reported

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Use of Gelatin Coating Solution 0.2%) is required for cell adhesion to the culture vessels. Grow cells in gelatin-coated culture vessels with the following conditions.
The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) PCR

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C