abm | GRPR-Luciferase Stable PC3 Cell Line | T3021

abm | GRPR-Luciferase Stable PC3 Cell Line | T3021

The PC3 prostate cancer cell line is widely used to represent a highly aggressive form of prostate cancer. The GRPR-PC3-Luciferase Stable Cell Line has been stably transfected with pcDNA3.1/hygro/Luc705, resulting in the insertion of the Luc705 cassette. The Luc705 cassette is a Firefly Luciferase coding sequence containing an aberrant intron; which prevents the correct expression of the Luciferase gene. Removal of the aberrant by various mechanisms - such as a splice shifting oligonucleotide (Oligonucleotide Treatment) - allows for proper translation of the gene, resulting in bioluminescence of the cell. The GRPR-PC3-Luciferase Stable Cell Line is useful for studying intracellular delivery of oligonucleotides and oligonucleotdie-conjugates via receptor mediated targeting.

Biosafety:

II

Organism:

Human

Source Organ:

prostate

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm

Preservation:

N/A

Quality Control:

1) Measured cellular uptake of peptide through flow optometry analysis (Figure 2A). 2) Analysed luciferase activity of peptide compared to controls via RT-PCR (Figure 2B&C).

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C