abm | HEK293S-TetR GnTI- Stably Expressing A160T Cell Line | T3165

abm | HEK293S-TetR GnTI- Stably Expressing A160T Cell Line | T3165

HEK293S-TetR GnTI Stably Expressing A160T Cell line was established to have a tetracycline-inducible HEK293S stable cell line which expresses the A160T mutant of the thromboxane A2 receptor (TP). In which the genetic variant exhibited loss of thermal stability when compared to the wild type, hence some loss of activity or thermal sensitivity.. Additionally, the HEK293S-TetR GnTI- cells do not express the N-acetylglucosaminyltransferase I (GnTI).TP is a G-protein coupled receptor (GPCR) involved in vasoconstriction and thrombosis. It is valuable tool for discovery of the TP biology, and the roles and functions of the receptor.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Thromboxane A2 receptor (TP) via transfection of FLAG-TP-A160T-1D4 plasmid

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

Immunodetection was used to detect TP genetic variant A160T

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C