Description
abm | HIF HRE-Luc-LacZ Stable Expressing Me1290 Human Uveal Melanoma Cell Line | T6296
HIF HRE-Luc/LacZ Stable Expressing Me1290 Human Uveal Melanoma Cell Line expresses hypoxia-responsive dual LacZ/Luciferase reporter to monitor HIF-1 activity. This cell line is useful to study the effects of HIF-1 activity.
Biosafety:
II
Organism:
Human (H. sapiens)
Source Organ:
Oral
Growth Properties:
Adherent
Morphology:
Epithelial-like
Clones:
N/A
Passage Number:
N/A
Population Doner:
30 - 40 hours
Seeding Density:
N/A
Markers:
Hygromycin B resistant
Applications:
For Research Use Only
Doner Gender:
Female
Donor Ethnicity:
Not disclosed
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
Note: selection antibiotics should not be added upon thawing or for routine cell propagation. Let cells recover first after thawing, then once they grow well put for 7 days in selection medium. Then grow again in normal medium.
The base medium for this cell line is Prigrow II medium available at abm
Preservation:
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
N/A
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C