abm | Luciferase Stable IGR-CaP1 Cell Line | T6102

abm | Luciferase Stable IGR-CaP1 Cell Line | T6102

Luciferase Stable IGR-CaP1 Cell Line was derived from primary prostate cancer. To obtain the IGR-CaP1 cells, a retrovirus was used expressing the luciferase gene (pMEGIX-Luc vector), carrying an expression cassette encoding the firefly luciferase and green fluorescent protein (GFP) genes. The GFP-positive IGR-CaP1 luciferase-expressing cells were sorted for green fluorescence before amplification. The IGR-CaP1 cell line is a unique model derived from a primary tumor, which can reconstitute human prostate adenocarcinoma in animals and generate experimental bone metastases, providing a novel means for understanding the mechanisms of bone metastasis progression and allowing preclinical testing of new therapies.

Biosafety:

II

Organism:

Human

Source Organ:

Prostate

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

GFP, luciferase

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Bioluminescence

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C