abm | NTCP Stable HepG2 Cell Line | T6190

abm | NTCP Stable HepG2 Cell Line | T6190

NTCP Stable HepG2 Cell Line was generated by infecting HepG2 cells with the lentivirus NT-GFP (pLVX-IRES-ZsGreen), in which GFP-expressing cells were enriched with FACS. NTCP (sodium taurocholate cotransporting polypeptide) is HBV receptor, making this cell line permissive to HBV. This cell line may be used to study HBV or the life cycle of hepatitis B, C and D viruses which utilizes NTCP for host entry.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Liver

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

NTCP

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) FACS

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C