Description
abm | OATP1B3 Stable Expressing HEK293 Cell Line | T6239
A new organic anion transporting polypeptide (OATP) was cloned and expressed; deemed human OATP2. OATP plays an important role in the uptake of a variety of lipophilic anions into the human liver. HEK293 cells were transfected with the genes to express the recombinant OATP2 protein onto the lateral membrane of the cells. OATP1B3 Stable Expressing HEK293 Cell Line presents a good model to study the molecular mechanism behind the uptake of organic anions such as bilirubin conjugates and sulfobromophthalein into the human liver.
Biosafety:
II
Organism:
Human (H. sapiens)
Source Organ:
Kidney
Growth Properties:
Adherent
Morphology:
Epithelial
Clones:
N/A
Passage Number:
N/A
Population Doner:
30 - 40 hours
Seeding Density:
50,000 - 70,000 cells/cm2; cells may take up to 72 hours to recover post-thaw
Markers:
OATP1B3 expression, G418 resistance
Applications:
For Research Use Only
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
The base medium for this cell line is Prigrow VII medium available at abm
Preservation:
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
1) qRT-PCR; 2) Immunoblot; 3) Immunofluorescence analyses
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C