abm | SIRT7 Stable Scramble HeLa Cell Line | T6258

abm | SIRT7 Stable Scramble HeLa Cell Line | T6258

SIRT7 Stable Scramble HeLa Cell Line was generated by lentiviral driven expression of shRNAs. Lack of Sirt7 is associated with reduced recruitment of DNMT1 and Sirt1 at rRNA genes leading to hyperacetylation of histones, reduced DNA methylation, fragmentation of the nucleolar structure and loss of rDNA repeats.

Biosafety:

II

Organism:

Human

Source Organ:

Cervix

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

10,000 - 20,000 cells/cm²

Markers:

Puromycin resistance

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C