abm | Stable 293T-ATD cell line | T6340

abm | Stable 293T-ATD cell line | T6340

Anti‐N‐methyl‐D‐aspartate Receptor Encephalitis (ANRE) is a life threatening autoimmune disease where the body makes antibodies that bind to NMDA receptors (NMDAR) in the brain. This disease manifests as a variety of neurological symptoms including seizures, hallucinations, and loss of motor function. The hippocampus and amygdala are the most affected centres of the brain. When anti-NMDAR antibodies bind to NMDA receptors there is a decrease in synaptic currents in the affected neurons. The Stable 293T-ATD cell line expressing NMDAR has been used extensively to uncover the molecular mechanisms that cause ANRE. This cell line would be ideal for research into NMDAR channel function and anti-NMDA antibodies associated with ANRE pathogenesis.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Advanced DMEM. To make the complete growth medium, supplement base media with the following components: heat inactivated fetal bovine serum to a final concentration of 1%, Penicillin/Streptomycin Solution

Preservation:

Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C