abm | Stable HEK293 Expressing Human NLRP3 Cell Line (huNLRP3-YFP) | T6318

abm | Stable HEK293 Expressing Human NLRP3 Cell Line (huNLRP3-YFP) | T6318

Stable HEK293 Expressing Human NLRP3 Cell Line (huNLRP3-YFP) were established through stable lipofectamine transfection of the YFP-tagged human NLRP3 gene to parental HEK293 cells. Monoclonal selection via 1 mg/ml G418 was conducted to maintain a selection pressure. The cell line established may be used to study NLRP3 inflammasome, which is highly expressed in macrophages, and its association with the inflammatory pathway.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 34 hours

Seeding Density:

4,000 cells/cm²

Markers:

NLRP3, Neomycin resistance (0.3 mg/ml G418)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Expression of NLRP3 detection by Western blot using anti-NLRP3 receptor antibody (Adipogen, clone Cryo-2); 2) YFP fluorescence

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C