abm | Stable Human ER Positive Breast Epithelial Cell Line | T6021

abm | Stable Human ER Positive Breast Epithelial Cell Line | T6021

Stable Human ER Positive Breast Cells expresses endogenous estrogen receptors (ER) in culture. It is a viable cell culture system enabling end users to conduct studies with oestrogen and screening of drug candidates suitable for breast cancer. The cells are a reliable source of renewable culture as the cells is immortalized with hTERT, thus limits batch to batch variation and gives expression of the estrogen receptors to manipulate.

Biosafety:

II

Organism:

Human

Source Organ:

Mammary tissue

Growth Properties:

Adherent

Morphology:

Epithelial-Like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

10,000 - 20,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

Infected with replication-incompetent lentivirus carrying the pBABE-neo-hTERT vector

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm

Preservation:

N/A

Quality Control:

1) FACS used to isolate ER-positive expressing breast cells; 2) Immunpcytochemical staining used to detect the presence of ER; 3) 500 μg/ml G418 used for selection

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C