abm | Stable TIM3 Expressing CHO-K1 Cell Line | T6121

abm | Stable TIM3 Expressing CHO-K1 Cell Line | T6121

CHO-K1 Cells were transfected with HAVCR2 Lentiviral Vector (pLenti-GIII-CMV-GFP-2A-Puro) (LV710795) to generate the Stable TIM3 Expressing CHO-K1 Cell Line. The cells were selected against puromycin-resistance and display adherent growth property with epithelial morphology. The Stable TIM3 Expressing CHO-K1 Cell Line is also positive for GFP-expression. The cells can be used in studies focusing on HAVCR2 gene and the TIM3 pathway with its interaction with the PD1 pathway. Other applications for the Stable TIM3 Expressing CHO-K1 Cell Line include positive control for western blot or binding assays.

Biosafety:

II

Organism:

Chinese hamster

Source Organ:

Ovary

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

15 - 20 hours

Seeding Density:

5,000 - 15,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfection with HAVCR2 Lentiviral Vector (pLenti-GIII-CMV-GFP-2A-Puro)

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is PriGrow IV medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Puromycin selection; 2) GFP-Screening

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C