abm | T2R14 Expressing HEK293T Cell Line | T6141

abm | T2R14 Expressing HEK293T Cell Line | T6141

T2R14 Expressing HEK293T Cell Line was generated through the cloning of TAS2R gene into pcDNA 3.1+ (Hygromycin) vector between KpnI and NotI restriction sites. T2R14 Expressing HEK293T Cell Line allows for the structural and functional analysis of T2R receptors. Possible applications of T2R14 Expressing HEK293T Cell Line would be the development of bitter compound blockers or antagonists for these receptors.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

70 - 80 hours

Seeding Density:

20,000 - 50,000 cells/cm²

Markers:

Hygromycin resistance, expression of FLAG sequence at N-terminus of bitter taste receptors

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is PriGrow IV medium available from abm TM004). To make the completed growth medium, add the following components to the base medium: Fetal Bovine Serum TM999) to a final concentration of 10% and Penicillin/Streptomycin G255) to a final concentration of 1%. Change medium every 2 days and split cells accordingly when the cells are at 80-90% confluent.
Carbon dioxide CO₂): 5%, Temperature: 37.0°C.

Preservation:

N/A

Quality Control:

Expression of FLAG sequence at N-terminus of receptors analyzed by flow cytometry.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C