abm | U87MG Stably Expressing Tet-off Cell Line | T6035

abm | U87MG Stably Expressing Tet-off Cell Line | T6035

U87MG cells were transfected with the tTA expression vector to generate the U87MG Stably Expressing Tet-off Cell Line. It can be used in vitro for transfected with other tetracycline-responsive expression vectors to create doxycycline-inducible gene expression cells for research. In the presence of doxycycline will turn off gene expression under the tet-regulated promoter.

abm also offers the tet-on cell line, U87MG Stably Expressing Tet-on Cell Line (T6032).

Biosafety:

II

Organism:

Human

Source Organ:

Brain

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfection with the tTA expression vector

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

Selection with 400 ug/ml G418

Tumorgenicn:

Yes

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C