abm | UBG76V-GFP Stable SiHa Cell Line | T6274

abm | UBG76V-GFP Stable SiHa Cell Line | T6274

UBG76V-GFP Stable SiHa Cell Line are derived from SiHa cells stably transfected to express ubiquitin mutant (UbG76V) and the GFP reporter. These cells are applicable for studies investigating the relationship between HPV E6/E7 proteins and ubiquitin-proteasome system.

Biosafety:

II

Organism:

Human

Source Organ:

Cervix

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

18 - 26 hours

Seeding Density:

5,000 cells/cm²

Markers:

GFP, G418 resistance (0.8 mg/mL)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot performed with anti-GFP antibodies (Cell Signaling Technology, #2555)

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C