Description
Cord blood iPSCs induced with non-viral EBNA1-based episomal vector pEV-SFFV-OS. All cell lines are tested to be free of any transgene vector insertion by PCR analysis and thus are authentic footprint-free iPSCs. These lines have all been passaged long-term (up to P30) without differentiation in optimized human iPSC culture media in the presence of inactivated REF or MEF feeder cells. These lines may have greater propensity to differentiate into Hematopoietic Stem Cells (HSCs) or other mesoderm cells due to epigenetic memory. Great cell model systems for any investigative research work including on iPSCs, HSCs, and disease modeling.
Vector:
pEV-SFFV-Oct4-2A-Sox2
Species:
Human
Organ:
Cord Blood
Passages:
10
Propagation:
The basal medium for this cell line is Prigrow X series medium available at abm, Cat. No TM2010. Carbon dioxide (CO2): 5%, Temperature:37.0°C.
Concentration:
10⁶ cells/vial
Induction Method:
N/A
Pluripotency:
N/A
Growth Properties:
N/A
Morphology:
N/A
Markers:
N/A
Subculturing:
N/A
QC:
N/A
Safety:
BioSafety Level II
Shipping:
Dry Ice
Storage:
-180°C
1 Review
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iPSC culture
I have been culturing these cells for a long time, and my observations are as follows: 1) I cultured the cells as soon as they arrived, which were sent with dry ice, and the cells were already adhered to Matrigel the next day. 2) Although some cells underwent differentiation during long-term culture, the cells could be cultured healthily after passaging. 3) We tested for mycoplasma, and the result was negative.