MOUSE IGG2B ISOTYPE CONTROL ANTIBODY:BIOTIN (A-1)

Mouse IgG2b isotype control antibody is supplied as a purified monoclonal antibody conjugated to biotin.

PRODUCT DETAILS – MOUSE IGG2B ISOTYPE CONTROL ANTIBODY:BIOTIN (A1)

• Mouse anti-Chicken IgA monoclonal IgG2b antibody (clone A1), conjugated to biotin and suitable for use as a negative control.
• Greater than 95% purity by SDS-PAGE and buffered in borate buffered saline pH8.2.

BACKGROUND

Isotype controls are a type of negative control designed to measure the level of non-specific background signal caused by primary antibodies. The background signal is often the result of immunoglobulins binding non-specifically to Fc receptors present on the cell surface. For example, antibodies raised in mice may bind to some human cells regardless of the test antibody specificity, in a manner related to the isotype of the antibody being used. In these cases you need an isotype control of the same isotype as the test antibody to provide a relevant control for non-specific binding.