AffiAB® Goat Anti-HIF1a Polyclonal IgG Antibody

Goat Anti-Human HIF1a IgG Polyclonal AntibodyAB0112

Overview: Goat polyclonal to alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in apoptosis, angiogenesis, energy metabolism, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in tumour angiogenesis, embryonic vascularization, and pathophysiology of ischemic disease.

Other Names: alpha subunit (basic helix-loop-helix transcription factor), ARNT interacting protein, basic-helix-loop-helix-PAS protein MOP1, bHLHe78, class E basic helix-loop-helix protein 78, HIF-1alpha, HIF1, HIF1-ALPHA, HIF1a, hypoxia inducible factor 1, hypoxia-inducible factor 1 alpha isoform I.3, hypoxia-inducible factor 1, hypoxia-inducible factor 1-alpha, member of PAS protein 1, member of PAS superfamily 1, MOP1, PASD8, PAS domain-containing protein 8 antibody.

Other Names: alpha subunit (basic helix-loop-helix transcription factor), ARNT interacting protein, basic-helix-loop-helix-PAS protein MOP1, bHLHe78, class E basic helix-loop-helix protein 78, HIF-1alpha, HIF1, HIF1-ALPHA, HIF1a, hypoxia inducible factor 1, hypoxia-inducible factor 1 alpha isoform I.3, hypoxia-inducible factor 1, hypoxia-inducible factor 1-alpha, member of PAS protein 1, member of PAS superfamily 1, MOP1, PASD8, PAS domain-containing protein 8 antibody.

Concentration: 3 mg/ml

Antibody Type: Primary

Target Type: Anti-HIF1a

Accession Number: ENSG00000100644

Host Species: Goat

Immunogen Species: Human

Immunogen Type: Recombinant protein

Immunogen: Purified recombinant peptide derived from within residues 780 aa to the C-terminus of human HIF1a produced in E. coli.

Isotype: IgG

Clonality: Polyclonal

Confirmed Reactive Species: Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Specificity Detail: Detects HIF1a in transfected cells and a band of approximately 120 kDa by Western blot in cell lysates.

Buffer: PBS, 20% glycerol and 0.05% sodium azide

Purification: Epitope affinity purified

Conjugation: Unconjugated

Format: N/A

Application: WB

Concentration/Dilution: WB:1:500-1:2,000

Storage:

References: 1. Ferreira JV, Rosa Soares A, Ramalho JS, et al. PLoS One. 2019 Oct. PMID:31613922, 2. Rodrigues TDA, PhD Thesis, University of Coimbra, Portugal 2018, 3. Fonseca LMO, MSc Thesis, University of Berlin, Germany 2018, 4. Rodrigues T, Matafome P, Sereno J, et al. Sci Rep. 2017 May. PMID:28490763, 5. Ferreira JV, Soares AR, Ramalho JS, et al. Scientific Reports 5, May 2015. PMID: 25958982, 6. Sacramento JF, Ribeiro MJ, Rodrigues T, et al. Respir Physiol Neurobiol. 2016 Jul. PMID:26993367