AffiAB® Goat Anti-RFP, DyLight® 633 Polyclonal IgG Antibody

The AffiAB® Goat Anti-RFP, DyLight® 633 Polyclonal IgG Antibody is an antibody specifically designed to target and bind to RFP (Red Fluorescent Protein) when conjugated with DyLight® 633, a near-infrared fluorescent dye. This antibody is produced by immunizing goats with RFP protein or peptides, and the resulting polyclonal antibody is purified and conjugated with the DyLight® 633 dye.

RFP is a commonly used fluorescent protein derived from Discosoma sp. coral, which emits red fluorescence when excited by appropriate wavelengths of light. By conjugating the anti-RFP antibody with DyLight® 633, the resulting antibody can be excited by red or near-infrared light and emit a near-infrared fluorescence signal when bound to RFP-tagged proteins.

The AffiAB® Goat Anti-RFP, DyLight® 633 Polyclonal IgG Antibody is particularly useful in experiments where the detection of RFP-tagged proteins needs to be performed in the near-infrared range. This wavelength range offers advantages such as reduced autofluorescence and deeper tissue penetration, making it suitable for applications such as in vivo imaging, deep tissue imaging, and multiplexing with other fluorescence channels.

When working with this antibody, it is important to follow the recommended protocols provided by the manufacturer, including sample preparation, antibody dilution, and incubation times. Proper controls and optimization steps should be performed to ensure specific binding and minimize background signals.

In summary, the AffiAB® Goat Anti-RFP, DyLight® 633 Polyclonal IgG Antibody is a valuable tool for researchers studying RFP-tagged proteins who require near-infrared fluorescence detection. It enables specific visualization and analysis of RFP-tagged proteins in various experimental setups, including those involving deep tissue imaging and multiplexing applications.