MOUSE ANTI-HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) P24 (1947)

Mouse anti HIV-1 p24 (clone 1947) antibody has been developed for use in ELISA and IFA.

PRODUCT DETAILS – MOUSE ANTI-HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) P24 (1947)

• Mouse anti Human Immunodeficiency Virus p24 (clone 1947).
• Specific for HIV-1 p24. Functions in ELISA and IFA.
• Purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations with greater than 90% purity.
• Presented in 0.01 M phosphate buffered saline, pH 7.2 with 0.1% sodium azide.

BACKGROUND

The human immunodeficiency virus HIV-1 establishes persistent infections in humans which lead to acquired immunodeficiency syndrome (AIDS). HIV-1 continues to be a major global health threat and the development of an effective vaccine is highly desirable to eradicate HIV/AIDS. Most existing viral vaccines work through the induction of neutralizing antibodies (NAbs) that block infection and/or viremia (Plotkin, 2010). However, despite great efforts, to date no HIV-1 vaccine has been developed that elicits broadly neutralizing antibodies (bNabs) to protect against infection. High antigenic diversity and densely populated N-linked glycan sites, which cover nearly the entire HIV-1 envelope protein (Env), are major roadblocks for the development of bNAbs by vaccination (van Schooten and van Gils, 2018).

P24 protein is a component of the HIV particle capsid which folds to form two domains connected by a flexible linker, providing multiple options for assembly. The onset of symptoms of AIDS correlates with increased levels of virus and p24 in the blood and a reduction in the number of CD4 T-cells which can be detected as early as 2 weeks after HIV infection using immunoassays. Decrease in the level of antibodies to p24 and p24 antigen has been considered to be one of the most useful prognostic markers for use in assays. Fourth-generation tests can now detect both HIV antibodies and p24 antigens, whereas older versions only looked for antibodies, resulting in a quicker diagnosis compared to previous generation tests. Most diagnostic kits to detect HIV antibodies incorporate p24, in addition to envelope glycoproteins.