Human Apolipoprotein CII (Apo CII), 20% glycerol | ABMC-P13

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Description

Human Apolipoprotein CII (Apo CII), 20% glycerol | ABMC-P13

Concentration:

0.7 mg / ml, determined by the Lowry method

Source:

From fresh human plasma that has tested negative for Hepatitis C, HIV-I and HIV-II antibodies as well as Hepatitis surface antigens.

Purification:

After series ultracentrifugations, Very Low Density Lipoprotein (VLDL) is isolated from human plasma. Apo CII is purified from delipidated VLDL, followed by gel-filtration and DEAE-Sepharcyl chromatography.

Purity:

≥ 98% by SDS-PAGE

Buffer:

In 10 mM PBS, 0.15 M NaCl, 0.02 % NaN3, 1mM EDTA, pH 7.2, with 20% glycerol

Storage:

-20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing.

*The products are for research or manufacturing use only, not for use in human therapeutic or diagnostic applications.

IMPORTANCE

Apo CII contains 78 amino acid residues. The m.w. is 8.5 kDa (Jackson et al., 1977).

The lipid-binding domain of Apo CII is localized to a region in the N-terminal with a strongly amphipathic nature (Macphee et al., 1999). Apo CII is also one of the key components of the metabolism of total triacylglycerol-rich lipoproteins by activating lipoprotein lipase that hydrolyzes fatty acids from triacylglycerols in chylomicrons (Kei et al., 2012).

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