Human Low Density Lipoprotein (LDL), 50% glycerol | ABMC-P08
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Description
Human Low Density Lipoprotein (LDL), 50% glycerol | ABMC-P08
| Concentration: | 1 mg / ml, determined by the Lowry method |
| Source: | From fresh human plasma that has tested negative for Hepatitis C, HIV-I and HIV-II antibodies as well as Hepatitis surface antigens. |
| Purification: | After series ultracentrifugation, Low Density Lipoprotein (LDL) is isolated from human plasma (Density: 1.063). |
| Purity: | ≥ 98% by SDS-PAGE |
| Buffer: | 20 mM Tris-HCl, 140 mM NaCl, 0.02% NaN3, 0.5 mM EDTA, pH 7.4; Preserved with 50 % glycerol. |
| Storage: | -20°C for long-term storage, 4°C for short- term storage. Aliquot to avoid repeated freezing and thawing. |
IMPORTANCE
The process of LDL formation begins when intrahepatic cholesterol is repackaged by the liver into VLDL (Very Low-density lipoprotein). VLDL then enters the circulation and is converted by lipoprotein lipase and cholesteryl ester transfer protein (CETP) into more cholesterolenriched species, first IDL (Intermediate-density lipoprotein) and then LDL (Wadhera et al., 2015).
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