LPS Detection Kit

LPS Detection Kit - Cat Number: 6039 From Chondrex.

Research Field: Bacterial Research

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: 0.31 ng/ml-20 ng/ml

Sample Type: Serum, Plasma

Concentration: N/A

Immunogen:

PRODUCT SPECIFICATIONS

DESCRIPTION: ELISA kit to quantify E. coli (O111:B4) LPS levels in samples
FORMAT: Pre-coated 96-well ELISA Plate with removeable strips
ASSAY TYPE: Sandwich ELISA
ASSAY TIME: 5.5 hours
STANDARD RANGE: 4 ng/ml to 0.06 ng/ml
NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate
SAMPLE TYPES: Serum and Plasma
RECOMMENDED SAMPLE DILUTIONS: 1:1 (at least)
CHROMOGEN: TMB (read at 450 nm)
STORAGE: -20°C
VALIDATION DATA: Intra-assay (2.6-6.6%)/Inter-assay (4.6-7.7%)/Spiking Test (106-110%)
NOTES:

INTRODUCTION

Lipopolysaccharide (LPS) is a major component of the outer-membrane in gram-negative bacteria. While the main role of LPS is to maintain
the integrity of the bacteria envelope, LPS also binds to the CD14/TLR4/MD2 receptor complex on many cell types (1,2), such as B cells,
macrophages, and dendritic cells, resulting in pro-inflammatory cytokine release from these cells (3). This activation of the innate immune
response causes inflammation which may play important roles in obesity and chronic inflammatory diseases such as Crohn’s Disease,
Chronic Fatigue Syndrome, Ulcerative Colitis, and Rheumatoid Arthritis. Translocation of bacteria toxins from intestinal flora may also trigger
the pathogenesis of autoimmune diseases (4,5). To examine the possible link between pathogenic toxins from intestinal bacteria and
autoimmune diseases, Chondrex Inc. provides an LPS (E. coli O111:B4) detection kit as well as ELISA kits for detecting staphylococcal
enterotoxins A and B (Catalog # 6029 & 6030). For further requests and consultation, please visit www.chondrex.com or contact Chondrex,
Inc. Customer Support at support@chondrex.com.
NOTE: Because E. coli (O111:B4) may not be a natural resident in SPF mouse guts, inoculation of the E. coli or its LPS (i.e. immunization
or oral feeding) may be required for animal studies. Chondrex, Inc. recommend testing LPS levels in naive animals to determine baseline
LPS levels that can result from different vendors, animal strains, and housing conditions.

KIT COMPONENTS

ASSAY OUTLINE

PLATE LAYOUT

NOTES BEFORE USING ASSAY

NOTE 1: It is recommended that the standard and samples be run in duplicate.
NOTE 2: Warm up all buffers to room temperature before use.
NOTE 3: Crystals may form in Wash Buffer, 20X when stored at cold temperatures. If crystals have formed, warm the wash buffer by placing
the bottle in warm water until crystals are completely dissolved.
NOTE 4: Measure exact volume of buffers using a serological pipet, as extra buffer is provided.
NOTE 5: Cover the plate with plastic wrap or a plate sealer after each step to prevent evaporation from the outside wells of the plate.
NOTE 6: For partial reagent use, please see the assay protocol’s corresponding step for the appropriate dilution ratio. For example, if the
protocol dilutes 50 µl of a stock solution in 10 ml of buffer for 12 strips, then for 6 strips, dilute 25 µl of the stock solution in 5 ml of buffer.
Partially used stock reagents may be kept in their original vials and stored at -20⁰C for use in a future assay.
NOTE 7: This kit contains animal components from non-infectious animals and should be treated as potential biohazards in use and for
disposal.