Mouse Anti-Porcine Type II Collagen IgG2b Subtype Antibody Assay Kit, TMB

Mouse Anti-Porcine Type II Collagen IgG2b Subtype Antibody Assay Kit, TMB - Cat Number: 20333T From Chondrex.

Research Field: Arthritis, Immunology

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: 10 ng/ml-0.16 ng/ml

Sample Type: Serum, Plasma

Concentration: N/A

Immunogen:

DESCRIPTION: Assay kit to quantify mouse anti-collagen antibodies

FORMAT: Pre-coated 96-well ELISA Plate with removeable strips
ASSAY TYPE: Indirect ELISA
ASSAY TIME: 4.25 hours
STANDARD RANGE: IgG1, IgG2a, IgG2b, and IgG2c : 10-0.16 ng/ml
IgG3 : 100-1.6 ng/ml
NUMBER OF SAMPLES: Up to 39 (duplicate) samples/standard plate (will vary for custom kits)
SAMPLE TYPES: Serum and Plasma
RECOMMENDED SAMPLE DILUTIONS: 1:1000 (at least)
CHROMOGEN: TMB (read at 450 nm)
STORAGE: -20°C
VALIDATION DATA: N/A

INTRODUCTION

Autoantibodies to type II collagen play a primary role in the collagen induced arthritis (CIA) model. However, autoantibodies are not always capable of inducing arthritis due to the inability to activate a complement, the first critical step in the activation of inflammatory cascades. In CIA susceptible DBA mice, complement fixable IgG2a and IgG2b subtype autoantibody levels, as well as their epitope specificity, are pertinent in the induction of arthritis. In order to adequately study the pathological roles of antibodies to type II collagen, it is important to determine individual IgG subtype autoantibody levels to mouse type II collagen as well as the heterologous type II collagen used for immunization.

On the other hand, C57BL/6 mice, a popular strain for developing transgenic mice, produce IgG2c antibodies instead of IgG2a antibodies due to its MHC class I haplotype (H-2b). Moreover, these mice can only develop CIA when immunized with chick type II collagen. Therefore, serum IgG2c autoantibody levels against mouse type II collagen and chick type II collagen should be evaluated when using C57BL/6 mice for the CIA model. Chondrex, Inc. provides Mouse Anti-Collagen IgG Antibody Subtype (IgG1, IgG2a, IgG2b, IgG2c, and IgG3) ELISA Kits for further analysis of antibodies in mouse serum from the mouse CIA model.

Depending on the IgG subtypes and epitopes, the antibody-antigen affinity varies significantly among individual antibodies and serum samples from individual animals. Therefore, the total IgG concentration calculated as the sum of individual IgG subtypes might not perfectly match the total IgG levels determined by Chondrex, Inc’s Mouse Anti-Collagen IgG ELISA Kit. These kits can also be used to determine antibody levels to type I collagen (chick, bovine, porcine, human, and mouse) upon request. Please contact Chondrex, Inc. at support@chondrex.com for more information on custom type I collagen-coated plates.

KIT COMPONENTS

PLATE COATING AND SETUP

ASSAY OUTLINE