ELISA Grade Human Type II Collagen, 0.5 mg/ml x 1ml

ELISA Grade Human Type II Collagen, 0.5 mg/ml x 1ml - Cat Number: 2015 From Chondrex.

Research Field: Arthritis, ECM

Clonality: N/A

Cross-Reactivity:

Host Origin: N/A

Applications: N/A

Isotype: N/A

Detection Range: N/A

Sample Type: N/A

Concentration: 0.5 mg/ml

Immunogen:

PRODUCT SPECIFICATIONS

DESCRIPTION: Highly purified type II collagen (treated with 3M guanidine, DEAE-cellulose, and Na2HPO4)
APPLICATION: Use as an ELISA antigen to detect antibodies specific to human type II collagen in sera from
experimental animals and humans, such as collagen-induced arthritis (CIA) models and patients
with arthritis or other diseases, as well as normal controls to study the diversity of anti-collagen
antibodies.
QUANTITY: 1 ml
FORM: 0.5 mg/ml solution in 0.05M acetic acid
SOURCE: Human sternal cartilage
MOLECULAR WEIGHT: 300 kDa
PURITY: >99% by SDS-PAGE, free of pepsin and proteoglycans
USAGE: Chondrex, Inc. recommends diluting this product with cold 1X Collagen Dilution Buffer, (10X
Collagen Dilution Buffer, Cat # 9003 as provided with this product), to prevent collagen
molecules from aggregating. For plate coating, take aliquots of ELISA grade collagen and dilute
them with cold 1X Collagen Dilution Buffer just before use. Do not keep diluted collagen
solutions. The optimum collagen concentration to coat ELISA plates is 5 µg/ml, but this may
vary based on the assay or type of plate used, such as cell culture plates.
STORAGE: -20°C. This product is stable in acidic conditions at 4°C for several weeks or at -20°C in the
dark for one year as supplied but will gradually form polymeric collagen and degrade under
neutral conditions.
STABILITY: 1 year
NOTES: N/A
REFERENCES: R. Trelstad, et al. Biochemistry 9: 4993-4998 (1970)
J. Seyer, et al. Clin. Invest. 59: 241-248 (1977)
K. Terato, et al. Arthritis Rheumatism 33: 1493-1500 (1990)
K. Terato, et al. Clin. Immunol. Immunopathol. 79: 142-154 (1996)