Barbiturate Group Forensic ELISA Kit | 130619

Barbiturate ELISA (Enzyme-Linked Immunosorbent Assay) kit is a qualitative one-step kit designed for use as a screening test for the detection of Barbiturates. The kit was designed for screening purposes and is intended for forensic use only.

Cross Reactivity

Materials Provided

1. EIA Buffer
2. Wash Buffer Concentrate (10X)
3. K-Blue Substrate
4. Drug-Enzyme Conjugate
5. Antibody Coated Plate
6. Acid Stop Solution
7. Whole Blood Cut-off Calibrators
   1. Blood Negative Calibrator
   2. Blood Cut-off Calibrator
8. Urine Negative Control
   1. Urine Negative Calibrator
   2. Urine Cut-off Calibrator
9. Oral Fluid Cut-off Calibrators
   1. Oral Fluid Negative Calibrator
   2. Oral Fluid Cut-off Calibrator

Materials Required But Not Provided

1. Deionized water.
2. Precision pipettes that range from 10 μL - 1000 μL and disposable tips.
3. Graduated cylinder to dilute and mix wash buffer.
4. Plate cover or plastic film to cover plate during incubation.
5. Clean glassware (i.e. test tubes) to dilute samples.
6. Microplate reader with 450 nm filter.

Optional Materials

1. Microplate shaker.