Legionella pneumophila RT-PCR (CE) | B50-50FRT

Legionella pneumophila RT-PCR | B50-50FRT from Sacace Biotechnologies is available for delivery

Description:

General information: Real Time PCR test for quantitative detection of Legionella pneumophila

Target Disease Type: Respiratory Infections

Specific Application: Legionella pneumophila

Storage and Shipping : 4 weeks

Legionella pneumophila RT-PCR (CE) B50-50FRT DataSheet

INTRODUCTION

Legionella pneumophila is a thin, pleomorphic, flagellated Gram-negative bacterium of the genus Legionella. L. pneumophila is the primary human pathogenic bacterium in this group and is the causative agent of legionellosis or Legionnaires' disease.

Legionella pneumophila (named in memory of the deceased veterans) is ubiquitous to acquatic environments worldwide and resided as an intracellular parasite of amoeba and protozoa provided a link between natural environment and human disease. Thus, environmental monitoring, especially of potable water, cooling towers, and related sources, is a major focus in efforts to control the spread of this disease.

Since the initial identification of 235 cases in 1976, Legionnaires disease has become recognized as the most common cause of atypical pneumonia in hospitalized patients. It is the second most common cause of community-acquired bacterial pneumonia with 25% mortality rate.

INTENDED USE

kit Legionella pneumophila Real-TM is a test for Real Time quantitative detection of Legionella pneumophila DNA in the clinical materials (sputum, aspirate from trachea, nasopharyngeal swabs, throat swabs, bronchoalveolar lavage, tissue), microorganism cultures, and qualitative detection and quantitation of Legionella pneumophila DNA in environmental samples (water, washes from environmental objects, biofilms scrapes, ground).

PRINCIPLE OF ASSAY

kit Legionella pneumophila Real-TM is a Real-Time Amplification test for the qualitative and quantitative detection of Legionella pneumophila in biological materials and in environmental samples.

Legionella pneumophila Real-TM kit can be used as:

• a qualitative test for Legionella pneumophila DNA detection in the clinical materials. During the test multiplex real-time PCR of Legionella pneumophila mip-gene DNA and protrombin gene DNA is performed. Protrombin gene DNA is used as endogenous internal control. Legionella pneumophila mip-gene DNA amplification is detected on JOE/Yellow channel, while the protrombin gene DNA amplification is detected on FAM/Green channel. Protrombin gene DNA is a part of human genome DNA and it should be present in adequate amount in DNA sample (no less then 103 genomes). Both improper storage conditions and poor DNA isolation process can lead to DNA degradation and loss. So, the endogenous internal control allows not only to control analysis steps, but also to estimate sample handling and storage.
• а qualitative test for Legionella pneumophila DNA detection in environmental samples. In this case the Internal Control (IC) is used. Legionella pneumophila mip-gene DNA amplification is detected on JOE/Yellow channel, while the IC DNA amplification is detected on FAM/Green channel.
• а quantitative test for Legionella pneumophila DNA calculation in water. In this case the Internal Control (IC) is used. Legionella pneumophila mip-gene DNA amplification is detected on JOE/Yellow channel, while the IC DNA amplification is detected on FAM/Green channel. To quantify Legionella pneumophila and Internal Control DNA copies quantitative standards are used.