Swine Influenza Virus A/H1 RT-PCR (CE) | V55-FRT

Swine Influenza Virus A/H1 RT-PCR (CE) | V55-FRT from Sacace Biotechnologies is available for delivery

Real-Time PCR test for the qualitative detection of Swine Influenza Virus A/H1 RNA

Storage & Shipping :

Transport Medium Plus should be transported at 2–25 °C..

Swine Influenza Virus A/H1 RT-PCR (CE) V55-FRT DataSheet

INTRODUCTION

Swine Influenza Virus (SIV) H1N1 (referred to as “swine flu” early on) is a new influenza virus causing illness in people. This new virus was first detected in people in Mexico, United States and Canada in April 2009. Other countries, including many European countries, have reported people sick with this new virus. This virus is spreading from person-to-person and it is different from seasonal H1N1 virus, which is a human one that usually circulates widely.

Real-time RT-PCR test for influenza A virus with “Influenza A,B Real-TM” (Sacace, ref. TV36-50FRT) is recommended as a screening test for all suspected cases of swine influenza A (H1N1) virus. Confirmation of swine-origin influenza A (H1N1) virus must be performed with Swine Influenza Virus A/H1 Real-TM Real-Time RT-PCR test. World Health Organization (WHO) in the guidance for the surveillance of human infection with swine influenza A (H1N1) virus recommend to perform real-time RT-PCR assay with specific SIV primers as a confirmatory test for swine influenza A virus.

INTENDED USE

Swine Influenza Virus A/H1 Real-TM is Real-Time amplification test for the qualitative detection of Swine Influenza Virus A/H1 RNA in clinical specimens.

PRINCIPLE OF ASSAY

Swine Influenza Virus A/H1 Real-TM Test is based on three major processes: isolation of virus RNA from specimens, reverse transcription of the RNA, Real Time amplification of the cDNA. Influenza virus A/H1-swine detection by the polymerase chain reaction (PCR) is based on the amplification of pathogen genome specific region using specific primers and detection via fluorescent dyes. These dyes are linked with probes of oligonucleotides which bind specifically to the amplified product. The real-time PCR monitoring of fluorescence intensities allows the accumulating product detection without reopening of reaction tubes after the PCR run. Swine Influenza Virus A/H1 Real-TM PCR kit is a qualitative test which contain the Internal Control (IC). It must be used in the isolation procedure in order to control the process of each individual sample extraction and serves also to identify possible reaction inhibition.