This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody. Abcam does not test ab34710 with endogenous samples in WB. We do recommend to look at the guidelines for blotting large proteins.
ab34710 has <5% cross-reactivity with Collagen III
Customers have been successful using ab34710 in this application, please see references below (Tillgren V et al.JBiolChem290:918-25; 2015).
Positive Control: Hu stomach, skin and adrenal gland tissue lysates.
Anti-Human Type I Collagen Antibody, Clone 222b - Cat Number: 7086 From Chondrex.
Research Field: ECM
Clonality: Monoclonal
Cross-Reactivity: B, H, Ms, P
Host Origin: Rat
Applications: ELISA, IHC,...
Anti-Human Type I Collagen Antibody, Clone 42r - Cat Number: 7025 From Chondrex.
Research Field: ECM
Clonality: Monoclonal
Cross-Reactivity: H
Host Origin: Rat
Applications: ELISA, IHC
Isotype:...