Description
Indole-3-Butyric Acid (IBA) Solution is a plant growth regulator widely used to promote root growth in plant tissue culture applications.
The Benefits
- Easy to use
- Use to supplement tissue culture media, such as MS media.
- Stimulates adventitious root development and callus formation.
- Often used with other hormones to promote optimal plant growth and development.
How To Use Indole-3-Butyric Acid (IBA) Solution
Follow the given steps to prepare the tissue culture media using PCT’s supreme grade agar:
- Prepare liquid tissue culture media using PCT’s MS, DKW media, or any other alternative media based on your plant.
- Add sugar based on the quantity of media you’re making (generally, 30 grams of sugar is added for 1-liter media), 1 ml/L PPM, and 3-4 grams per liter of gellan gum or 6-8 grams per liter of agar.
- Then, add the desired quantity of the plant hormone.
- Autoclave the medium for 20 minutes at 15 psi and 121℃.