Goat Anti-PGC1A/PPARGC1A Antibody | EB07856

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SKU:: EB07856
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Availability:: In Stock
Applications:: Pep-ELISA, WB, IF, FC
Tested species:: Human, Rat
Size:: 100µg specific antibody in 200µl

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Description

Goat Anti-PGC1A/PPARGC1A Antibody | EB07856

Target Protein

Principal Names: PGC1A, PPARGC1A, peroxisome proliferator-activated receptor gamma, coactivator 1 alpha, LEM6, PGC-1(alpha), PGC-1v, PGC1, PPARGC1, PPAR gamma coactivator variant form, PPAR gamma coactivator-1, ligand effect modulator-6, peroxisome proliferative activated receptor, gamma, coactivator 1, peroxisome proliferative activated receptor, gamma, coactivator 1, alpha
Official Symbol: PPARGC1A
Accession Number(s): NP_037393.1; NP_001317680.1; NP_001317681.1; NP_001317682.1; NP_001341756.1;
Human GeneID(s): 10891
Non-Human GeneID(s): 19017 (mouse), 83516 (rat)

Immunogen

Peptide with sequence C-DGLFDDSEDESDK, from the internal region of the protein sequence according to NP_037393.1; NP_001317680.1; NP_001317681.1; NP_001317682.1; NP_001341756.1;.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 90kDa band observed in Rat Stomach lysates and in preliminary teting of Mouse Stomach lysate (calculated MW of 90.6kDa according to Rat NP_112637.1. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the nucleus and weaker expression in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.