Description
HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) | BA1054
Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate, for the indirect sensitive immunodetection and/or quantification of target proteins through WB, or ELISA by assaying an HRP-catalyzed reaction product in the vicinity of the antigen-primary antibody-secondary antibody-HRP complex.
Reagent Type | Secondary antibody, reporter enzyme labeled |
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Label | HRP (Horseradish Peroxidase) |
Host | Goat |
Target Species | Rabbit |
Antibody Class | IgG |
Clonality | Polyclonal |
Immunogen | Rabbit IgG (whole molecular) |
Purification | The antibody was purified from antisera by immunoaffinity chromatography. |
Specificity | This HRP conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG. |
Form Supplied | Concentrated, Liquid |
Formulation | 0.5 mg of HRP conjugated specific antibody 0.01 M PBS (PH 7.4) 50% glycerol |
Pack Size | 0.5 ml |
Concentration | 1mg/ml |
Application | ELISA*, WB* *Our Boster Guarantee covers the use of this product in the above marked tested applications. |
Storage | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. |
Precautions | FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE |
Assay Information
Sample Type | SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates |
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Assay Type | Immunoassay |
Assay Purpose | Protein detection/quantification |
Technique | Immunodetection of target antibody with HRP reporter enzyme |
Equipment Needed | WB/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer |
Compatibility with Reagents | Incompatible with sodium azide and metals incompatible with high phosphate concentrations |