MicroVue SC5b-9 Plus EIA | A029

The MicroVue SC5b 9 Plus Enzyme Immunoassay measures the amount of the SC5b 9 complex present in human plasma or serum specimens.

The Terminal Complement Complex (TCC, SC5b-9) is generated by the assembly of C5 through C9 as a consequence of activation of the complement system by either the classical, lectin or alternative pathway. The membrane attack complex (MAC), a form of TCC, is a stable complex that mediates the irreversible target cell membrane damage associated with complement activation. Complexes formed in the absence of a target membrane bind to naturally occurring regulatory serum proteins, e.g. the S protein,5-7 at the C5b 7 stage of assembly forming, soluble, non-lytic TCC.

The MicroVue SC5b 9 Plus Enzyme Immunoassay measures the concentration of TCC thereby giving an indication of the status of the terminal complement pathway in the specimen. It uses a monoclonal antibody to the C9 ring of TCC to capture the complex. The trapped TCC is subsequently detected with HRP-conjugated antibodies that bind to antigens of the SC5b 9 complex. This test, which provides a rapid, highly specific and quantitative procedure for measuring TCC levels, is designed for investigations studying the role or status of terminal complement pathway activation in numerous research settings.

Product Specifications

Scientific Validation

• Citations: 97 peer-reviewed publications

Kit Format

• Size: 96 wells / test
• Form: 96-well microplate with 12 removable 8-well strips, supplied in a resealable foil pouch

Sample Requirements

• Specimen:

                -Serum: 10 µL

                -EDTA or Citrated Plasma: 50 µL

Analytical Performance

• Limit of Detection (LOD): 3.7 ng/mL
• Lower Limit of Quantification (LLOQ): 8.8 ng/mL
• Upper Limit of Quantification (ULOQ): Not applicable

Precision

• Intra-Assay CV: 1.6–6.8%
• Inter-Assay CV: 5.0–13.1%

Calibration & Controls

• Standards: 5
• Controls: 2

Expected Sample Values

• Not established

Assay Conditions

• Total Assay Time: Approximately 2 hours

Cross-Reactivity

• African Green Monkey
• Baboon
• Cynomolgus Monkey
• Pigtail Monkey
• Rabbit
• Rhesus Monkey

Storage

• Store unopened kits at 2°C to 8°C
• Refer to the Product Insert for additional storage and stability information

Regulatory Status:
For Research Use Only (RUO). Not for use in diagnostic procedures.