NT-proBNP ELISA Product Overview

The NT-proBNP ELISA kit is a 3.5 hour, 96-well sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of NT proBNP in human serum and EDTA plasma.

NT-proBNP ELISA Assay Principle

The NT pro BNP ELISA is a sandwich enzyme immunoassay for the quantitative determination of NT-proBNP in human serum and EDTA plasma.

The figure below explains the principle of a sandwich ELISA:

In a first step, sample and conjugate (sheep anti-human NT-proBNP-HRP) are pipetted into the wells of the microtiter strips, which are pre-coated with polyclonal sheep anti-NT-proBNP antibody. NT-proBNP present in the sample binds to the pre-coated antibody in the well and forms a sandwich with the conjugate (detection antibody). In the washing step all non-specific unbound material is removed. In a second step, the substrate (Tetramethylbenzidine; TMB) is pipetted into the wells. The enzyme catalyzed color change of the substrate is directly proportional to the amount of NT-proBNP present in the sample. This color change is detectable with a standard microtiter plate ELISA reader.

The NT-proBNP ELISA kit uses highly purified, epitope-mapped antibodies.  The antibodies utilized in the NT-proBNP ELISA (BI-20892) are as follows:

Capture antibody: AA 32-57 (polyclonal sheep anti-human NT-proBNP)
Detection antibody: AA 8-29 (HRP-labeled polyclonal sheep anti-human NT-proBNP)