Protein A/G Magnetic Beads for IP | B23201

Advantages

• Spinning free, IP (takes<30 minutes) with minimal sample loss.
• Background caused by non-specific binding is very low.
• Beads contain 9.3×10¹³ molecules/cm² of protein A/G. Antibody binding capacity up to 0.4-0.5 mg/mL.
• Cheap to 73 USD/mL

Protein A/G Magnetic Beads for IP use a biological nanosurface technology (S-TEC). Protein A/G is orientated as a coat on the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10¹³ molecules/cm². Compared to other similar immune magnetic beads, Bimake MagBeads™ Protein A/G display more antibody binding sites, therefore during IP, less magnetic beads are used. Non-specific binding is low, enabling Bimake MagBeads Protein A/G to be used in IP conveniently and efficiently. With a large, specific surface area, these beads can greatly shorten the equilibrium antibody and antigen adsorption time, enabling complete antibody antigen adsorption process within 10 minutes, and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.

Specificity

Protein A/G is a recombinant fusion protein that combines IgG binding domains of both Protein A and Protein G. Protein A/G contains four Fc binding domains from Protein A and two from Protein G, yielding a final mass of 50,460 daltons. The binding of Protein A/G is less pH-dependent than Protein A, but otherwise has the additive properties of Protein A and G. Table1 provides a reference of binding strength between antibodies and protein A/G, use the antibody subtype with strong binding effect in your experiment.

Properties

Storage

Store at 4°C for 2 years. Don’t freeze in the absence of glycerol.