Kligler's Iron Agar | DM137D

Kligler's Iron Agar | DM137D

For the differentiation of the Enterobacteriaceae on the basis of double sugar fermentation and hydrogen sulphide production.

MAST Kligler's Iron Agar is based on Bailey and Lacey's modification of the original Kligler formulation. They recommended the use of phenol red indicator, to give enhanced differentiation of enteric organisms. Kligler's Iron Agar is reported to give more reliable H2S results than Triple Sugar Iron Agar (DM224), with certain sucrose fermenting organisms

Typical Formula

Directions

1. Suspend 55.6g in 1 litre or the contents of the sachet in the stated volume of distilled or deionised water.
2. Bring to the boil and dissolve completely.
3. Mix well and distribute.
4. Autoclave at 121°C (15 p.s.i.) for 15 minutes.
5. Allow to set as slopes with 1 inch butts.

Typical Result

A = Acid K= Alkaline N/C= No Change.
Symbols enclosed in parentheses indicate occasional reactions.

In Use

Kligler's Iron Agar should be used in conjunction with MAST Urea Agar Base (DM228) for differentiating non lactose fermenting colonies from selective media. Smear the surface of the Kligler slope and stab the butt with a single colony or pure subculture. Incubate for 18-48 hours at 37°C.