Description
MacConkey Agar (without salt) | DM140
A selective medium for the isolation and identification of enteric bacteria.
MAST MacConkey Agar without salt is a selective and differential identification medium. It is used for the isolation of enteric
bacteria from faeces and is used in water testing. Cultivated organisms are differentiated on the basis of their lactose fermentation, which is demonstrated by pink colonies and non-lactose fermentation, by white or colourless colonies. A highly purified and carefully selected grade of bile salts is used to give good inhibition of nonenteric bacteria and excellent survival of enteric organisms. The medium will support the growth of most staphylococci. Colour differentiation is good, and enterococci
stand out clearly. The absence of salt from the medium suppresses the swarming of most Proteus spp.
2. Technical Formula*
3. Directions
1. Suspend by swirling 44.5g of powder in 1 litre or the contents of the sachet in the stated volume of distilled or deionised water.
2. Autoclave at 121°C (15 p.s.i.) for 15 minutes.
3. Mix well before pouring.
4. In Use
Colony characteristics after 18 hours incubation.