PD-1 Antibody [F42D7] | SD8645

PD-1 Antibody [F42D7]

Specifications

• HOST SPECIES: Llama
• SPECIES REACTIVITY: Human
• IMMUNOGEN: PD-1 antibody was raised against a recombinant protein corresponding to amino acids 25 - 167 of human PD-1.
• CONJUGATE: Unconjugated
• TESTED APPLICATIONS: E, Flow
• APPLICATION NOTE: PD-1 antibody can be used for detection of PD-1 by ELISA and FACS (10 μg/ml).

Properties

• PURIFICATION: PD-1 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.It also contains a myc-tag for detection.
• CLONALITY: Recombinant Monoclonal
• ISOTYPE: sdAb
• PHYSICAL STATE: Liquid
• BUFFER: PD-1 Antibody is supplied in PBS.
• CONCENTRATION: 1 mg/mL
• STORAGE CONDITIONS: PD-1 antibody should be stored in working aliquots at -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

• NCBI OFFICIAL SYMBOL: PDCD1
• ADDITIONAL NAMES: PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1
• Protein Accession Number: NP_005009
• PROTEIN GI NUMBER: 145559515
• NCBI GENE ID NUMBER: 5133
• USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

• PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.
• 1: Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
• 2: Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
• 3: Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.
• 4: Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.