PD-L1 Antibody [F6A9] | SD8639

PD-L1 Antibody [F6A9]

Specifications

• HOST SPECIES: Llama
• SPECIES REACTIVITY: Human
• IMMUNOGEN: PD-L1 antibody was raised against a recombinant protein corresponding to amino acids 19 - 239 of human PD-L1.
• CONJUGATE: Unconjugated
• TESTED APPLICATIONS: Flow, ICC
• APPLICATION NOTE: PD-L1 antibody can be used for detection of PD-L1 by Flow Cytometry and Immunocytochemistry at 10 μg/ml.
• SPECIFICITY: At least three isoforms of PD-L1 are known to exist.

Properties

• PURIFICATION: PD-L1 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.It also contains a myc-tag for detection.
• CLONALITY: Recombinant Monoclonal
• ISOTYPE: sdAb
• PHYSICAL STATE: Liquid
• BUFFER: PD-L1 Antibody is supplied in PBS.
• CONCENTRATION: 1 mg/mL
• STORAGE CONDITIONS: PD-L1 antibody should be stored in working aliquots at -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

• NCBI OFFICIAL SYMBOL: CD274
• ADDITIONAL NAMES: PD-L1 Antibody: B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, B7 homolog 1
• Protein Accession Number: NP_054862
• PROTEIN GI NUMBER: 7661534
• NCBI GENE ID NUMBER: 29126
• USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

• PD-L1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts.
• 1: Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
• 2: Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
• 3: LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.