AffiGEL® Matrix High Concentration, LDEV-Free Equivalent to: CORNING Matrigel Cat No. 354248

AffiGEL® Matrix High Concentration, LDEV-Free

Equivalent: 354248 | Corning® Matrigel® Basement Membrane Matrix High Concentration (HC), LDEV-free

Matrix type: High concentration (≥18mg/ml)

Application: Experiments such as angiogenesis, gel embolization, and in vivo tumor formation (for angiogenesis,it is recommended that the final concentration of AffiGEL® basement membrane matrix should be ≥10mg/ml)

Product Information 

AffiGEL® Matrix High Concentration, LDEV-Free is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Source

Engelbreth-Holm-Swarm Mouse Tumor

Quality

• Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
• Extensive PCR testing is performed to screen for a number of pathogens
• Tested and found negative for bacteria, fungi, and mycoplasma
• Protein concentrations determined by Lowry method
• Endotoxin units measured by Limulus Amoebocyte Lysate assay
• Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
• Biological activity is determined for each lot using a neurite outgrowth assay

Preparation and Storage

Store at -20°C. Avoid multiple freeze-thaws.

Application

• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• in vitro and in vivo Angiogenesis Assays
• in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

AFG-YSG-10

AFG-YSG-11