Annexin V-FITC Apoptosis Detection Kit

Annexin V-FITC Apoptosis Detection Kit | 15342-54 from Nacalai Tesque is available for delivery.

• Annexin V-FITC Apoptosis Detection Kit contains ready-to-use solutions, Annexin V-FITC conjugate, propidium iodide (PI).
• The kit can identify apoptotic and necrotic cells
• Detect by flow cytometry or fluorescence microscopy
• No need to fix cells

Product Information

Applications

Cell type: Mouse B16 melanoma cells
Number of cells: 10,000
Instrument: Gallios (Beckman Coulter Inc.)

Apoptosis was induced by cycloheximide (1 µg/ml) or UV irradiation at 37°C for 3.5 hours. Cells were analyzed by flow cytometry. Our product showed about the same performance as the product from company A. The control cells did not display any signs of apoptosis. Treatment with cycloheximide resulted in many cells in early apoptosis. UV irradiation also induced apoptosis in the tested cells.

--: FITC-labeled Annexin V and PI both had low fluorescence values. Live cells.
+-: FITC-labeled Annexin V had high fluorescence value, PI was low. Early apoptosis.
++: FITC-labeled Annexin V and PI both had high fluorescence values. Late apoptosis.