DBH (Dopamine-beta-Hydroxylase) Antibody | 22806

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SKU:
484-22806
Quantity/Volume:
100 µL
  • DBH (Dopamine-beta-Hydroxylase) Antibody | 22806
  • IHC image of neurons staining for DBH in the rat brainstem. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
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Description

DBH (Dopamine-beta-Hydroxylase) Antibody | 22806 | ImmunoStar

The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/2000 – 1/4000 dilution in rat brainstem, cerebellum and adrenal medulla. The above image is of DBH positive neurons in rat brainstem, vibratome sectioned, ABC / DAB detection. Using Western blot of purified DBH the antiserum detects a triplet at approximately 72-74 kD. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Host: Rabbit

State: Lyophilized Whole Serum

Species Reactivity: Bird, Cat, Ferret, Finch, Guinea Pig, Hamster, Hatchetfish, Human, Ilyanassa Obsoleta (Sea Snail), Monkey, Mouse, Pig, Quail, Rat, Sparrow, Starling, Steer (Cattle), Turkey, Turtle, Zebra Finch

Availability: In Stock

Alternate Names: DBM; DOPO; Dopamine beta-monooxygenase, anti-DBH

RRID: AB_572229

Immunogen: Bovine DBH

Gene Symbol: DBH

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