Protein Electrophoresis

Protein electrophoresis is a fundamental analytical technique in molecular biology and biochemistry. It enables the separation, identification, and quantification of proteins based on their size, charge, or conformation using an electric field applied to a gel matrix. As research advances in proteomics, diagnostics, and quality control, the demand for high-performance electrophoresis systems continues to grow.

This article provides an in-depth look into protein electrophoresis and presents a full range of electrophoresis systems.

What Is Protein Electrophoresis ?

Protein electrophoresis refers to the migration of proteins through a gel matrix (commonly polyacrylamide) under an electric field. Proteins are separated primarily by molecular weight (SDS-PAGE), isoelectric point (IEF), or both (2D electrophoresis).

Types of Protein Electrophoresis Systems

There are several electrophoresis methods tailored for different research needs:

1. SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis)

  • Denaturing method for size-based separation
  • Ideal for analyzing protein purity and molecular weight

2. Native PAGE

  • Non-denaturing separation
  • Maintains protein structure and activity

3. Isoelectric Focusing (IEF)

  • Separation based on isoelectric point (pI)
  • Useful for resolving proteins with subtle charge differences

4. Two-Dimensional Gel Electrophoresis (2D-GE)

  • Combines IEF and SDS-PAGE
  • High-resolution proteome profiling

Applications of Protein Electrophoresis

Protein electrophoresis is essential in:

  • Proteomics research
  • Clinical diagnostics (e.g., serum protein electrophoresis)
  • Western blotting (post-electrophoresis transfer)
  • Recombinant protein analysis
  • Pharmaceutical QC and drug purity assessment
  • Genetic research involving protein variants

Advanced Electrophoresis Systems by ATTO Corporation

With ATTO, we provide state-of-the-art electrophoresis and gel casting systems for protein separation, DNA/RNA analysis, and molecular biology workflows. Below is a curated overview of some of their most in-demand instruments, engineered for high-resolution and reproducibility in lab-scale and industrial-scale research.

1- WSE-1010 cPAGE Ace

Compact SDS-PAGE System for Protein Electrophoresis

  • Optimized for 10×10 cm mini gels
  • Integrated gel casting and running unit
  • Ideal for research laboratories with limited space

 

2- WSE-1025 cPAGE Ace Twin

Dual-Run Vertical Electrophoresis System

  • Run 2 gels simultaneously for high-throughput screening
  • Compact footprint with efficient cooling
  • Suitable for molecular weight profiling

   

3- WSE-1090 Multi Compact Gel Cast

Multi-Gel Casting Station

  • Cast multiple gels in parallel
  • Reduces prep time and increases lab productivity
  • Compatible with WSE-1010 / WSE-1025

    

4- WSE-1165 Mini-Slab

Mini Vertical Electrophoresis System

  • Designed for small-format PAGE analysis
  • Fast run times with clear band resolution
  • Supports native and denaturing PAGE

 

5- WSE-1150 PageRunAce

Integrated Electrophoresis & Gel Casting System

  • Plug-and-play solution for SDS-PAGE
  • Leak-proof chamber with easy handling
  • Clear protein separation with minimal heat generation

 

6- AE-6401 1mm Dual Mini Gel Cast

Precision Dual Gel Casting Station

  • Allows simultaneous casting of two 1 mm mini gels
  • Consistent thickness ensures reproducible results
  • Compatible with multiple vertical tanks

 

7- WSE-1190 Multi Mini-Slab Gel Cast

Parallel Casting for Mini Slab Gels

  • Fabricates up to four mini slab gels at once
  • Ideal for large sample series
  • Supports high-resolution protein studies

    

8- WSE-1170 Multi Lane Gel Chamber

Multi-Lane Electrophoresis Tank

  • Designed for comparative analysis across lanes
  • Customizable combs for multiple samples per gel
  • Supports multiplexed SDS-PAGE

 

9- AE-6210 Slab Gel Cast

Large Format Gel Casting Unit

  • Suitable for high-volume or preparative gels
  • Accurate casting thickness with tight sealing
  • Commonly used in proteomics and diagnostics

  

                                     

10- WSE-1510 DiscRun Ace

Disc Electrophoresis for Protein Fractionation

  • Unique disc gel format for zone separation
  • Fine resolution of protein bands
  • Compatible with specific analytical protocols

 

                             

Ideal for :

  • Academic labs
  • Biotech companies
  • Pharmaceutical QC teams
  • Clinical diagnostic R&D

All units are available with technical support, user manuals, and training resources.

Start Your Protein Electrophoresis with Confidence

Whether you're studying protein expression, validating molecular weight, or performing biomarker discovery, ATTO’s systems offer accuracy, efficiency, and ease of use.

? Shop or inquire now: Info@gentaur.com