PurKine™ His-Tag Ni-Super Packed Column | BMC20020

Product Overwiew:

PurKine™ His Tagged Protein Purification Ni-Super Packed Column

Background:

PurKine™ His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ His-Tag Purification resin is also available as prepacked spin column and kit formats.

Used for:

N/A

Kit components:

N/A

Features & Benefits:

• High capacity—More than 10 mg of 6xHis-tagged protein per milliliter of resin.
• High toleration—More tolerable to various chemicals including reducing, chelating agents.The nickel ions have been shown to remain bound to the chelating ligand even after incubation for 24 hours in 10 mM EDTA
• Versatile—For purification under native or denaturing conditions.
• Cost-saving—No performance decrease after at least five repeated uses of the same batch of resin.
• Flexible—available in multiple formats including bulk resin, spin columns and complete kits.• Easy Cleaning—Directly use NaOH to cleaning-in-place.

Usage Notes:

N/A

Storage buffer:

Liquid resin in PBS buffer containing 20% ethanol were prepacked in columns.

Storage instructions:

Stable for one year at 2-8°C from date of shipment. Do not freeze.

Shipping:

Blue ice

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.