Hybrid-Q Plasmid Prep Mini provides two methods for easy and rapid preparation of plasmid DNA from the mini scale bacterial cells. Plasmid DNA can be prepared from up to 10 ml of overnight culture by conventional miniprep method with standard protocol. Alternatively, up to 3 ml of sample can be processed by rapid protocol in just 10 minutes with new patented EzClearTM filter and simultaneous processing of multiple samples can be easily performed. Up to 30 ㎍ of pure plasmid can be purified using Hybrid-Q Plasmid Rapidprep kit and this pure plasmid DNA is ready for PCR, cloning, fluorescent sequencing, synthesis of labeled hybridization probes, cell transfection, electroporation and enzymatic restriction analysis without further manipulation.

Applications :

Plasmid DNA in small scale.

Key Features:

• Spin column format.
• Rapid purification with EzClearTM filter : complete in just 10 minutes.
• Stable and consistent result.
• Instant use : No need of additional materials.
• 30 ㎍ of binding capacity and high purity.
• Compatible with endA+ strains.
• No use of organic solvents.
• Ready for use in fluorescent sequencing, cloning, hybridization, electroporation and other enzymatic manipulation.

Storage Conditions:

Hybrid-Q Plasmid Rapidprep Kit is shipped at room temperature. All components are stable at room temperature until the date of expiration that is printed on the product label. After addition of RNase A, buffer S1 is stable for 1 year when stored at 4˚C. In cold ambient condition, buffer S2 and G3 may exhibit salt precipitation and this will cause reduction of DNA recover-yields. If so, heat the bottle with occasional swirling in 37 ˚C water bath until completely dissolved.