Retro-CAG-eGFP-RG was packaged in HEK293T cells with Ca3(PO4)2 transfection of 3 plasmids of pRetro-CAGGFP, pRetro-RC, and pCMV-RG. The pRetro-CAG-GFP was constructed by using the components of Moloney Murine Leukemia virus (MoMLV), a combination of the cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter (CAG), and enhanced green fluorescent protein (eGFP) expression cassette. The pCMV-RG is expressed the envelop protein of rabies virus. Retro-CAG-eGFP-RG is a replication deficient virus and has potentials to infect many types of cell and tissue. It belongs to biosafety level-1 (BSL-1). Retro-CAG-eGFP-RG is provided as concentrated viral supernatant at a titer around 106 IU/ml in HEK293T cells

Feature :

• Bright GFP expression: The Retro-CAG-eGFP-RG can express the green fluorescent protein brightly in many cultured cells and animals.
• Replication deficient virus: The Retro-CAG-eGFP-RG is replication deficient virus.

Applications :

• Neuroscience: The Retro-CAG-eGFP-RG can be used in nervous system.
• Virology: The Retro-CAG-eGFP-RG can be used as a control virus in virological researches.
• Gene therapy research: The Retro-CAG-eGFP-RG can be used as a control retroviral vector when the targeted gene expressed by using retroviral vector in neurological field.

Storage Conditions :

• The product is shipped on dry ice. Store at -80°C.