Description
TCBS Agar | 86348
Description générale
Thiosulfate citrate bile salts sucrose (TCBS) Agar uses an alkaline pH (8.6), ox bile and 1% NaCl to prevent the growth of gram positive bacteria, members of the Enterobacteriaceae family and the genus Pseudomonas.
Application
Thiosulfate citrate bile salts sucrose (TCBS) Agar is a selective agar for the isolation and cultivation of Vibrio cholerae and other Vibrio species.
Notes préparatoires
Dissolve 88 g in 1 litre distilled water and bring to the boil. Pour plates. Do NOT autoclave.
Composition
Agar, 14 g/L
Bromothymol blue, 0.04 g/L
Ferric citrate, 1 g/L
Mixed peptone, 10 g/L
Ox-bile, 5 g/L
Sodium chloride, 10 g/L
Sodium cholate, 3 g/L
Sodium citrate, 10 g/L
Sodium thiosulfate, 10 g/L
Sucrose, 20 g/L
Thymol blue, 0.04 g/L
Yeast extract, 5 g/L