Protein Quantification Kit (Bradford Assay) | KTD3002

Product Overwiew:

Abbkine Protein Quantification Kit (Bradford Assay) provides a simple and rapid procedure for determining the concentration of protein in samples.

Background:

The Bradford assay, a colorimetric protein assay, is based on an absorbance shift of the dye Coomassie Brilliant Blue G-250. The Coomassie Brilliant Blue G-250 dye exists in three forms: anionic (blue), neutral (green), and cationic (red). Under acidic conditions, the red form of the dye is converted into its blue form, binding to the protein being assayed. If there's no protein to bind, then the solution will remain brown. The cationic (unbound) form is green / red and has an absorption spectrum maximum historically held to be at 465 nm. The anionic bound form of the dye which is held together by hydrophobic and ionic interactions, has an absorption spectrum maximum historically held to be at 595 nm. The increase of absorbance at 595 nm is proportional to the amount of bound dye, and thus to the amount (concentration) of protein present in the sample.

Used for:

N/A

Kit components:

• Coomassie Assay Reagent
• Albumin Standard (BSA)

Features & Benefits:

• Time saving—almost immediate color development.
• Compatible—unaffected by most chemical substances. The concentration of mercaptoethanol and dithiothreitol in the samples can reach 1mM and 5 mM respectively.
• Broad range—working range from 50 to 1000 µg/ml.

Usage Notes:

Due to the influence of high concentration detergent, it is necessary to ensure that the concentration of SDS is lower than 0.1 %, Triton X-100 is lower than 0.1 % and Tween 20, 60, 80 is lower than 0.06 % in the sample.

Storage buffer:

N/A

Storage instructions:

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping:

Gel pack with blue ice.

Precaution:

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.